pspgrna vector (Addgene inc)
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Addgene inc
pspgrna vector
Pspgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspgrna+vector/pSPgRNA+(Plasmid+%2347108)/pm40010349-220-11-13
Average 96 stars, based on 164 article reviews
Pspgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspgrna+vector/pSPgRNA+(Plasmid+%2347108)/pm40010349-220-11-13
Average 96 stars, based on 164 article reviews
pspgrna vector - by Bioz Stars,
2026-10
96/100 stars
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Activation Assay:Article Title: Methionine and Leucine Promote mTOR Gene Transcription and Milk Synthesis in Mammary Epithelial Cells through the eEF1Bα-UBR5-ARID1A Signaling. Article Snippet: Amino acids are essential for the activation of the mechanistic target of rapamycin (mTOR), but the corresponding molecular mechanism is not yet fully understood.. We previously found that Met stimulated eukaryotic elongation factor α (eEF1Bα) nuclear localization in bovine mammary epithelial cells (MECs).. Herein, we explored the role and molecular mechanism of eEF1Bα in methionine (Met)and leucine (Leu)-stimulated mTOR gene transcription and milk synthesis in MECs. eEF1Bα knockdown decreased milk protein and fat synthesis, cell proliferation, and mTOR mRNA expression and phosphorylation, whereas eEF1Bα overexpression had the opposite effects. Article Title: Methionine Promotes Milk Synthesis through the BRCC36-BRG1-mTOR Signaling Axis in Mammary Epithelial Cells. Article Snippet: Methionine (Met) functions as a key stimulator on the mTOR signaling pathway and milk synthesis, but the molecular mechanism remains incompletely understood.. We investigated the regulatory roles of BRCC36 in Met-stimulated milk lipid and protein synthesis, cell proliferation, and the mTOR signaling pathway.. Knockdown of BRCC36 promoted milk lipid and protein synthesis in HC11 cells as well as cell proliferation by increasing the levels of mTOR gene transcription and protein phosphorylation. Construct:Article Title: Methionine and Leucine Promote mTOR Gene Transcription and Milk Synthesis in Mammary Epithelial Cells through the eEF1Bα-UBR5-ARID1A Signaling. Article Snippet: Amino acids are essential for the activation of the mechanistic target of rapamycin (mTOR), but the corresponding molecular mechanism is not yet fully understood.. We previously found that Met stimulated eukaryotic elongation factor α (eEF1Bα) nuclear localization in bovine mammary epithelial cells (MECs).. Herein, we explored the role and molecular mechanism of eEF1Bα in methionine (Met)and leucine (Leu)-stimulated mTOR gene transcription and milk synthesis in MECs. eEF1Bα knockdown decreased milk protein and fat synthesis, cell proliferation, and mTOR mRNA expression and phosphorylation, whereas eEF1Bα overexpression had the opposite effects. Article Title: ITGB2 is a central hub-gene associated with inflammation and early fibro-atheroma development in a swine model of atherosclerosis Article Snippet: The gRNAs oligonucleotides were synthesized by Integrated DNA Technologies (IDT Technologies, IA, USA). .. ITGB2 gRNA expression plasmids were constructed by ligating annealed gRNA oligonucleotides using T4 ligase (NEB, USA) to Article Title: ITGB2 is a central hub-gene associated with inflammation and early fibro-atheroma development in a swine model of atherosclerosis. Article Snippet: The gRNAs oligonucleotides were synthesized by Integrated DNA Technologies (IDT Technologies, IA, USA). .. ITGB2 gRNA expression plasmids were constructed by ligating annealed gRNA oligonucleotides using T4 ligase (NEB, USA) to CRISPR:Article Title: Methionine and Leucine Promote mTOR Gene Transcription and Milk Synthesis in Mammary Epithelial Cells through the eEF1Bα-UBR5-ARID1A Signaling. Article Snippet: Amino acids are essential for the activation of the mechanistic target of rapamycin (mTOR), but the corresponding molecular mechanism is not yet fully understood.. We previously found that Met stimulated eukaryotic elongation factor α (eEF1Bα) nuclear localization in bovine mammary epithelial cells (MECs).. Herein, we explored the role and molecular mechanism of eEF1Bα in methionine (Met)and leucine (Leu)-stimulated mTOR gene transcription and milk synthesis in MECs. eEF1Bα knockdown decreased milk protein and fat synthesis, cell proliferation, and mTOR mRNA expression and phosphorylation, whereas eEF1Bα overexpression had the opposite effects. Expressing:Article Title: ITGB2 is a central hub-gene associated with inflammation and early fibro-atheroma development in a swine model of atherosclerosis Article Snippet: The gRNAs oligonucleotides were synthesized by Integrated DNA Technologies (IDT Technologies, IA, USA). .. ITGB2 gRNA expression plasmids were constructed by ligating annealed gRNA oligonucleotides using T4 ligase (NEB, USA) to Article Title: Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates the ZNF334 gene to inhibit the growth of colorectal cancer. Article Snippet: Although therapeutic targets for colorectal cancer (CRC) treatment have been developed, the treatment outcomes are not ideal and survival rates for CRC patients remain low.. It is critical to identify a specific target and develop an effective CRC treatment system.. The ZNF334 gene is a newly identified member of Zinc-finger proteins (ZNFs), which is essential for key biological processes associated with tumorigenesis. Article Title: ITGB2 is a central hub-gene associated with inflammation and early fibro-atheroma development in a swine model of atherosclerosis. Article Snippet: The gRNAs oligonucleotides were synthesized by Integrated DNA Technologies (IDT Technologies, IA, USA). .. ITGB2 gRNA expression plasmids were constructed by ligating annealed gRNA oligonucleotides using T4 ligase (NEB, USA) to Clone Assay:Article Title: Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease Article Snippet: The viral vector was transduced to teloHAEC cells at MOI 20, screened by Blasticidin S HCL (Corning, Fisher Scientific# 15383671) selection (6 μg/mL) and stored in liquid nitrogen for future use. .. The guide RNAs were cloned into the Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: .. Preparation of sgRNA plasmid: sgRNAs, targeting different genes, were cloned into Article Title: Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease. Article Snippet: The viral vector was transduced to teloHAEC cells at MOI 20, screened by Blasticidin S HCL (Corning, Fisher Scientific# 15383671) selection (6 mg/mL) and stored in liquid nitrogen for future use. .. The guide RNAs were cloned into the Plasmid Preparation:Article Title: Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease Article Snippet: The viral vector was transduced to teloHAEC cells at MOI 20, screened by Blasticidin S HCL (Corning, Fisher Scientific# 15383671) selection (6 μg/mL) and stored in liquid nitrogen for future use. .. The guide RNAs were cloned into the Article Title: Interferon-responsive intestinal BEST4/CA7 + cells are targets of bacterial diarrheal toxins. Article Snippet: .. Preparation of sgRNA plasmid: sgRNAs, targeting different genes, were cloned into Article Title: Methionine Promotes Milk Synthesis through the BRCC36-BRG1-mTOR Signaling Axis in Mammary Epithelial Cells. Article Snippet: Methionine (Met) functions as a key stimulator on the mTOR signaling pathway and milk synthesis, but the molecular mechanism remains incompletely understood.. We investigated the regulatory roles of BRCC36 in Met-stimulated milk lipid and protein synthesis, cell proliferation, and the mTOR signaling pathway.. Knockdown of BRCC36 promoted milk lipid and protein synthesis in HC11 cells as well as cell proliferation by increasing the levels of mTOR gene transcription and protein phosphorylation. Article Title: Transcriptomic and spatial dissection of human ex vivo right atrial tissue reveals proinflammatory microvascular changes in ischemic heart disease. Article Snippet: The viral vector was transduced to teloHAEC cells at MOI 20, screened by Blasticidin S HCL (Corning, Fisher Scientific# 15383671) selection (6 mg/mL) and stored in liquid nitrogen for future use. .. The guide RNAs were cloned into the |